dopa2 medium (Biogems International)
Biogems International is a verified supplier 
94
Structured Review
Biogems International
dopa2 medium
Dopa2 Medium, supplied by Biogems International, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dopa2+medium/SAG/pmc07044800-68-27-37
Average 94 stars, based on 3 article reviews
Dopa2 Medium, supplied by Biogems International, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dopa2+medium/SAG/pmc07044800-68-27-37
Average 94 stars, based on 3 article reviews
dopa2 medium - by Bioz Stars,
2026-10
94/100 stars
Images
1) Product Images from "Peptide-Based Scaffolds for the Culture and Transplantation of Human Dopaminergic Neurons"
Article Title: Peptide-Based Scaffolds for the Culture and Transplantation of Human Dopaminergic Neurons
Journal: Tissue Engineering. Part A
doi: 10.1089/ten.tea.2019.0094
Figure Legend Snippet: Protocol for differentiation and encapsulation of DA neurons. (A) Protocol for expansion of DA neural progenitors and final differentiation of DA neurons. (B) Representative phase-contrast images of DOPA1, DOPA2, and DOPA3 cells. Scale bar: 100 μm. (C) Timeline for encapsulation within SAPNS. DA, dopaminergic; SAPNS, self-assembling peptide nanofiber scaffolds. Color images are available online.
Techniques Used:
Figure Legend Snippet: Characterization of DA neural progenitors and DA neurons. (A) Immunocytochemical staining of the following markers: Nestin: neural stem/progenitor, Tuj1 (β-III tubulin): neuron, MAP2: mature neuron, TH: DA neuron, Ki-67: proliferative cells, Synapsin: synaptic vesicle, FOXA2: midbrain DA neuron, PITX3: midbrain DA neuron, En-1 (Engrailed-1): midbrain DA neuron, DAPI: nucleus. Scale bar: 50 μm. (B) qRT-PCR analysis comparing gene expression in 2D and 3D (SAPNS-encapsulated) DA neurons at day 22 of differentiation. Data are presented as log2 of the fold change in expression relative to DOPA2 neural progenitors, shown in a bar graph (left) and heat map (right). Error bars represent standard deviation. p > 0.05, Student's unpaired t-test. 2D, two-dimensional; 3D, three-dimensional; qRT-PCR, quantitative reverse transcription-polymerase chain reaction; TH, tyrosine hydroxylase. Color images are available online.
Techniques Used: Staining, Quantitative RT-PCR, Expressing, Standard Deviation, Reverse Transcription Polymerase Chain Reaction
Related Articles
Staining:Article Title: Peptide-Based Scaffolds for the Culture and Transplantation of Human Dopaminergic Neurons Article Snippet: After 48 h, 0.5 μM Smoothened Agonist (SAG) was added to the medium and the concentration of EC-23 was lowered to 1 μM.μM Smoothened Agonist (SAG) was added to the medium and the concentration of EC-23 was lowered to 1 μM. ... Twenty-four hours later, neurospheres were plated onto a six-well tissue culture polystyrene (TCPS) dish (Eppendorf) ( Supplementary Figure S2 ) coated with 5 μg/mL laminin (MilliporeSigma), in DOPA2 medium (base medium supplemented with Quantitative RT-PCR:Article Title: Peptide-Based Scaffolds for the Culture and Transplantation of Human Dopaminergic Neurons Article Snippet: After 48 h, 0.5 μM Smoothened Agonist (SAG) was added to the medium and the concentration of EC-23 was lowered to 1 μM.μM Smoothened Agonist (SAG) was added to the medium and the concentration of EC-23 was lowered to 1 μM. ... Twenty-four hours later, neurospheres were plated onto a six-well tissue culture polystyrene (TCPS) dish (Eppendorf) ( Supplementary Figure S2 ) coated with 5 μg/mL laminin (MilliporeSigma), in DOPA2 medium (base medium supplemented with Expressing:Article Title: Peptide-Based Scaffolds for the Culture and Transplantation of Human Dopaminergic Neurons Article Snippet: After 48 h, 0.5 μM Smoothened Agonist (SAG) was added to the medium and the concentration of EC-23 was lowered to 1 μM.μM Smoothened Agonist (SAG) was added to the medium and the concentration of EC-23 was lowered to 1 μM. ... Twenty-four hours later, neurospheres were plated onto a six-well tissue culture polystyrene (TCPS) dish (Eppendorf) ( Supplementary Figure S2 ) coated with 5 μg/mL laminin (MilliporeSigma), in DOPA2 medium (base medium supplemented with Standard Deviation:Article Title: Peptide-Based Scaffolds for the Culture and Transplantation of Human Dopaminergic Neurons Article Snippet: After 48 h, 0.5 μM Smoothened Agonist (SAG) was added to the medium and the concentration of EC-23 was lowered to 1 μM.μM Smoothened Agonist (SAG) was added to the medium and the concentration of EC-23 was lowered to 1 μM. ... Twenty-four hours later, neurospheres were plated onto a six-well tissue culture polystyrene (TCPS) dish (Eppendorf) ( Supplementary Figure S2 ) coated with 5 μg/mL laminin (MilliporeSigma), in DOPA2 medium (base medium supplemented with Reverse Transcription Polymerase Chain Reaction:Article Title: Peptide-Based Scaffolds for the Culture and Transplantation of Human Dopaminergic Neurons Article Snippet: After 48 h, 0.5 μM Smoothened Agonist (SAG) was added to the medium and the concentration of EC-23 was lowered to 1 μM.μM Smoothened Agonist (SAG) was added to the medium and the concentration of EC-23 was lowered to 1 μM. ... Twenty-four hours later, neurospheres were plated onto a six-well tissue culture polystyrene (TCPS) dish (Eppendorf) ( Supplementary Figure S2 ) coated with 5 μg/mL laminin (MilliporeSigma), in DOPA2 medium (base medium supplemented with |